Production of Xylooligosaccharide from Cassava Pulp’s Waste by Endo-β-1,4-D-Xylanase and Characterization of Its Prebiotic Effect by Fermentation of Lactobacillus acidophilus

This study explores the production of prebiotic xylooligosaccharide (XOS) from cassava pulp waste and its effectiveness for growth Lactobacillus acidophilus (L. acidophilus). We successfully produced characterized XOS xylan using a Bacillus sp. endo-β-1,4-D-xylanase. The was added to modify MRS medium (MRSm) in various concentrations (0, 1, 3 5%) which L. inoculated. observed every 12 h 2 days, fermentation products were analyzed pH, sugar content, short-chain fatty acids (SCFA) terms types amount. showed that grew well MRSm. optimum concentration MRSm 5%, indicated by highest (8.61 log CFU mL−1). profile SCFA is 14.42 mM acetic acid, 0.25 propionic 0.13 isobutyric 0.41 n-butyric 0.02 n-valeric isovaleric 25.08 lactic acid.